Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the prefrontal (prelimbic/infralimbic) cortex of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group. ***: p < 0.001 (post hoc Duncan’s multiple range test).

Article Snippet: Primary antibodies included rabbit polyclonal antibodies against BDNF [Cat# N-20 sc-546,RRID:AB_63094, Santa Cruz Biotechnology, Heidelberg, Germany] and trkB [Cat# (794) sc-12,RRID:AB_632557, Santa Cruz Biotechnology], both diluted 1:1000, along with a mouse monoclonal antibody against PSA-NCAM (Cat# MAB5324, RRID:AB_95211, Merck Millipore, Darmstadt, Germany) also diluted 1:1000 in TBS containing 5% milk powder and 0.02% sodium azide.

Techniques: Western Blot

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the anterior cingulate cortex of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group. **: p < 0.01 (post hoc Duncan’s multiple range test).

Article Snippet: Primary antibodies included rabbit polyclonal antibodies against BDNF [Cat# N-20 sc-546,RRID:AB_63094, Santa Cruz Biotechnology, Heidelberg, Germany] and trkB [Cat# (794) sc-12,RRID:AB_632557, Santa Cruz Biotechnology], both diluted 1:1000, along with a mouse monoclonal antibody against PSA-NCAM (Cat# MAB5324, RRID:AB_95211, Merck Millipore, Darmstadt, Germany) also diluted 1:1000 in TBS containing 5% milk powder and 0.02% sodium azide.

Techniques: Western Blot

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the ventral hippocampus of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group. *: p < 0.05; **: p < 0.02 (post hoc Duncan’s multiple range test).

Article Snippet: Primary antibodies included rabbit polyclonal antibodies against BDNF [Cat# N-20 sc-546,RRID:AB_63094, Santa Cruz Biotechnology, Heidelberg, Germany] and trkB [Cat# (794) sc-12,RRID:AB_632557, Santa Cruz Biotechnology], both diluted 1:1000, along with a mouse monoclonal antibody against PSA-NCAM (Cat# MAB5324, RRID:AB_95211, Merck Millipore, Darmstadt, Germany) also diluted 1:1000 in TBS containing 5% milk powder and 0.02% sodium azide.

Techniques: Western Blot

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the dorsal hippocampus of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group.

Article Snippet: Primary antibodies included rabbit polyclonal antibodies against BDNF [Cat# N-20 sc-546,RRID:AB_63094, Santa Cruz Biotechnology, Heidelberg, Germany] and trkB [Cat# (794) sc-12,RRID:AB_632557, Santa Cruz Biotechnology], both diluted 1:1000, along with a mouse monoclonal antibody against PSA-NCAM (Cat# MAB5324, RRID:AB_95211, Merck Millipore, Darmstadt, Germany) also diluted 1:1000 in TBS containing 5% milk powder and 0.02% sodium azide.

Techniques: Western Blot